The length, width, thickness, length/width ratio and 100-seed weight of Magnolia officinalis seeds from 15 natural distribution regions in China were measured and their germination rates were tested. The results showed that there were significant differences among seeds from different region in seed characters and 100-seed weight. Correlation analysis between seed characters and geographical climatic factors showed that the main variation was of geographical locations. The annual mean temperature, annual precipitation and frost-free period were the leading ecological factors restricting the seed variation. The results of test showed that there were significant differences in germination rates among different seed sources. The seeds from Ningqiang had the highest germination rate, while that from Kaixian the minimum. The seeds from Jingning, Wuyishan and Longsheng had high germination potential. According to cluster analysis on 5 trait-location mean values including seed size and quality and geographical climatic factors, the Magnolia officinalis from 15 natural distribution regions could be divided into 2 provenance regions, i.e. south-east M. officinalis provenance region and north-west provenance region.

Systematic experiments concerningmorphological and physiological changes associated with senescence of cut Lycoris aurea were conducted. The results indicated thatwhen the concentration of CoCl2 was 40 or 80 mg L-1 , it could considerably extended the longevity of cut L. aurea, increasing vase life from 5 days ( under control) to 9 days and imp rovingthe quality of flowers effectively. Itwas concluded that this p reservative with 40 mg·L-1 could retard water stress, imp rovewater balance and delay senescence p rocess of cut L. aurea. CoCl2 not only slowed the rate of p rotein decomposition but also decreased inMDA content and increased peroxidase content. Moreover, the results also showed a good effect of CoCl2with 80 mg L-1 on cut L. aurea. So there was a great foreground for p reservation of cut L. aurea.

在红花石蒜不同生长发育期进行八分法切割繁殖,研究无性繁殖系数和子球生物学性状差异性。结果表明:(1)不同生长发育期无性繁殖系数在0. 80～6. 80之间浮动,休眠期的6、7月和旺盛生长初期的9月繁殖系数较高;(2)不同生长发育期无性繁殖的子球鲜质量、最大直径、子球高度、根系长度、根基粗度及根系数量等生物学性状均呈现1%水平极显著差异。综合分析认为休眠期的6、7月,旺盛生长初期的9月及生长后期的4月是适宜无性繁殖的季节。

利用ISSR分子标记技术对14个居群的红花石蒜进行遗传多样性研究,结果表明: POPGEN32分析显示红花石蒜物种的遗传多样性很高,多态位点百分率为92. 31% , Shannon指数(H)为0. 459 7,Nei指数( I)为0. 302 5;居群水平的遗传多样性较低,多态位点百分率平均为49. 65% , Shannon指数(H)平均为0. 262 0, Nei指数( I)平均为0. 176 3;居群间的遗传分化系数(Gst )为0. 503 5,基因流(Nm)为0. 698 3。AMOVA分子变异分析显示:居群间遗传分化程度高, 46. 12%的变异发生在居群内, 53. 88%的变异发生于居群间。生境的片段化使居群间的基因流受阻,可能是居群间高遗传分化和居群水平低遗传多样性的主要原因。

以红花石蒜叶片基因组DNA为模板,分析了模板DNA、引物、dNTPs、Mg2+的浓度及Taq DNA聚合酶的用量对ISSR-PCR扩增结果的影响。建立了石蒜ISSR分析最优化的反应体系及应用程序:即25μL反应体系中,有20 ng模板DNA、0.5μmol.L-1随机引物、150μmol.L-1dNTPs、2.0 mmol.L-1Mg2+、1.0 U Taq DNA聚合酶。反应程序为:94℃预变性5 m in;然后45个循环:每个循环94℃变性45 s,55℃退火60 s,72℃延伸2 m in;循环结束后72℃延伸7 m in。

目的 研究不同光质处理下三叶崖爬藤生长及有效成分含量变化，以确定适宜三叶崖爬藤生长的最适光质条件。 方法 以2年生三叶崖爬藤为材料，用不同颜色的滤光膜（红色、黄色、绿色、蓝色和白色）进行光质处理，测定三叶崖爬藤叶片的叶形指标、生理指标、生物量及块茎中总黄酮含量。 结果 表明：红光处理下的三叶崖爬藤在叶形指标、生理指标、生物量和总黄酮含量上均得到改善，而其余光质处理结果仅对其中某一指标有所改善。 结论 红光处理下的三叶崖爬藤块茎的产量和质量均较佳，在三叶崖爬藤人工栽培中可以利用红光提高其产量及有效成分含量。

目的 对2个回归样地内8个不同种源厚朴幼苗生长适应性进行评价，为厚朴野生资源回归保护提供科学依据。 方法 观测幼苗生长性状指标，并运用主成分分析和灰色关联分析评价其回归适应性。 结果 表明：（1）厚朴回归苗木与理想种关联度为0.7947；（2）1号回归样地（浙江磐安）和2号回归样地（浙江遂昌）与理想种关联度分别为0.7741和0.8154；（3）8个回归种源与理想种关联度由高到低分别为：浙江遂昌>浙江磐安>浙江安吉>福建光泽>杂交幼苗>江西庐山>福建建宁>江西铜鼓种源。 结论 厚朴野外回归幼苗总体长势良好，适应性较好，不同种源、同一种源在不同回归样地内生长差异较大，回归幼苗来源和回归地可能是影响其回归保护的关键因子

By crossing experiments of some camellia species and C.japonica cultivars from 2002 to 2003, the results showed that cross-compatibility among some camellia species crossing C.japonica cultivars were better than those among some camellia species crossing species. While crossing among camellia species, the average fruit set percentage of 19 cross-combinations were 18.18% in 2002 and 21.35% in 2003,the range of fruit set percentage were from 4.00% to 64.00%.

［目的］ 谷氨酰胺磷酸核糖焦磷酸转酰胺酶（ASE）在植物嘌呤合成中发挥着关键作用，本研究的目的是详细揭示杨树ASE蛋白的生化功能特性。［方法］ 通过半定量RT-PCR、蛋白质亚细胞定位以及测定纯化蛋白的催化活性等方法来研究其在体外的生化功能，进一步通过互补拟南芥缺失突变体（atase2）来研究杨树ASE基因的体内生物学功能。［结果］ 从杨树基因组中鉴别出2个ASE基因，序列分析发现它们之间有很高的蛋白质序列相似性。半定量RT-PCR分析发现两个杨树ASE基因在根、茎、叶和芽中均表达。蛋白质亚细胞定位分析发现杨树ASE蛋白均定位在叶绿体中，而且两个杨树ASE蛋白均能完全互补拟南芥atase2突变体的表型。酶学性质分析表明，杨树两个ASE蛋白均能催化5-磷酸核糖-α-焦磷酸生成5-磷酸核糖-β-胺。［结论］ 本研究预示着两个杨树ASE蛋白在嘌呤合成中均发挥着重要作用。